Real-time PCR quantification of Treponema denticola in subgingival plaque from patients with different severities of chronic periodontitis / 中国组织工程研究

BACKGROUND:Oral treponemes (especially Treponema denticola) are widely considered to play important roles in the occurrence and development of chronic peridontitis.Treponema denticola can be detected in both periodontal patients and healthy individuals,so its pathogenicity may depend on its relative numbers in subgingival plaque.OBJECTIVE:To detect the number of Treponema denticola in subgingival plaque of patients with different severities of chronic periodontitis and to investigate the relationship between the bacterial colonization and the periodontal status.METHODS:Subgingival plaque samples were respectively taken from 132 patients with chronic periodontitis and 40 healthy controls,admitted in the Department of Stomatology,Aviation General Hospital of Chinese Medical University from July 2015 to August 2016.Based on the clinical data,132 patients were divided into mild (n=41),moderate (n=46),and severe (n=45) groups.The presence,relative numbers and proportion of Treponema denticola in the samples were detected by TaqMan real-time PCR assay.RESULTS AND CONCLUSION:There was no difference in the presence of Treponema dentico/a in subgingival plaque between healthy and periodontal participants.The relative numbers and proportion of Treponema denticola in the periodontal patients were significantly higher than those in the healthy controls (P ＜ 0.000 1).The quantification of Treponema denticola in different stages of periodontitis did show significant differences (P ＜ 0.05).In addition,the bacterial level and proportion of Treponema denticola were positively correlated to the probing depths (P ＜ 0.000 1).When the probing depth was more than 7 mm,the bacterial level and proportion of Treponema denticola were significantly increased (P ＜ 0.05,P ＜ 0.01).These findings suggest that Treponema denticola widely exists in subgingival plaque of human.The bacterial load and proportion of Treponema denticola are tightly related to the severity of periodontitis and probing depth.The higher bacterial level and proportion of Treponema denticola indicate a severer periodontitis.Moreover,real-time PCR possesses a broad potential in the studies on etiology,diagnosis and treatment of pedodontology.

Roles of the central nucleus of amygdala in the cardiovascular response elicited by the paraventricular nucleus of hypothalamus / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To investigate the cardiovascular responses induced by activation of the paraventricular nucleus of hypothalamus (PVN) and the roles of the central nucleus of amygdala (CeA) on this effect.</p><p><b>METHODS</b>The PVN was activated by microinjection of L-glutamate or electrical stimulation. The CeA was injected with L-glutamate or Kainic acid (KA). The femoral arterial pressure, mean arterial pressure (MAP), electrocardiogram (ECG) and heart rate (HR) of the male SD rats were recorded when the PVN was electrically stimulated.</p><p><b>RESULT</b>The blood pressure increased when the PVN was activated either by electrical current or by L-glutamate. The blood pressure increased for (10.27+/-1.80)mmHg and the change of heart rate was -10.66 +/- 8.11 beat/min after L-Glu (100 nl) was injected into the ipsilateral CeA. The pressor response of PVN stimulation could still be evoked by electrical stimulation of (13.78 +/- 3.18)mmHg 10 min after kainic acid (100 nl) was injected into the ipsilateral CeA. But this pressor response decreased of 6.57 mmHg compared to that before injection of KA (P <0.05). The locations of the electrode tips and termination of the injector tracts were identified according to the atlas after the recording.</p><p><b>CONCLUSION</b>Stimulating the PVN elicits pressor response in rats. The CeA mediates partly the pressor response elicited by activation of the PVN.</p>

Role of dorsal column in pathway of hypotensive effect of the somatic afferent inputs / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To investigate the role of the dorsal column (DC) in the inhibitory effect of somatic afferent inputs on the central pressor response.</p><p><b>METHODS</b>The femoral arterial pressure, mean arterial pressure (MAP), electrocardiogram (ECG) and heart rate (HR) of the male SD rats were recorded when the hypothalamic paraventricular nucleus (PVN) was electrically stimulated with or without destruction of DC. The inhibitory effect of the deep peroneal nerve (DPN) on the pressor response induced by stimulation of PVN was observed 20 min or 5 d after ipsilateral DC destruction.</p><p><b>RESULTS</b>Stimulating DPN inhibited the pressor response elicited by electrical stimulation of PVN with an inhibitory rate of 43.29%. Twenty minutes after destroying the right DC, stimulation of the right or left DPN could inhibit the pressor response with an inhibitory rate of 38.64% and 39.97%, respectively (P>0.05); five days later the inhibitory rates remained as 33.87% and 36.86% respectively (P>0.05). The pain responses of both hindlimbs in the rats with the right DC destroyed showed no significant difference compared with the intact rats.</p><p><b>CONCLUSION</b>DC is not involved in the inhibitory effect of DPN on the pressor response induced by PVN stimulation.</p>

Study on matrix metalloproteinase 1, 9, 12 polymorphisms and susceptibility to chronic obstructive pulmonary disease among Han nationality in northern China / 中华流行病学杂志

<p><b>OBJECTIVE</b>To study the association between the functional polymorphism of matrix metalloproteinases (MMPs) and the development of chronic obstructive pulmonary disease (COPD).</p><p><b>METHODS</b>147 COPD patients and 120 healthy smoking controls were selected. Spirometry and chest X-rays had been taken. Questionnaires including sex, age, smoking history, occupational exposure were completed. MMP-9 (-1562 C/T), MMP-1(-1607 1G/2G), MMP-12 (-82 A/G), MMP-12(-357 Asn/ Ser) alleles were determined using PCR-RFLP method. Independent samples T test analysis was carried out to compare patients' age, smoking index, FEV1 /FVC, FEV1 % pred with that of healthy controlled group. The frequencies of genotypes and alleles between groups were analyzed by chi-square tests and multilogistic regression.</p><p><b>RESULTS</b>MMP12 Asn/Asn, CT/AsnAsn were risk factors for smoking-induced COPD. The ORs were 2.361 (95% CI: 1.369-4.017) and 2.433(95% CI: 1.159-5.342) respectively while CC/1G1G/ SerSer seemed to be a protective factor for smoking-induced COPD, with OR as 0.457 and 95% CI as 0.231-0.911.</p><p><b>CONCLUSION</b>Asn/Asn, CT/AsnAsn might be susceptible genotypes while CC/GG/SerSer might serve as protective genotype.</p>

The role of dorsomedial hypothalamic nucleus in the cardiovascular responses induced by habenular nucleus stimulation and inhibitory effect of deep peroneal nerve / 中国应用生理学杂志

<p><b>AIM</b>To explore whether dorsomedial hypothalamic nucleus (DMH) is involved in the cardiovascular responses induced by habenular nucleus (Hb) stimulation and inhibitory effect of deep peroneal nerve (DPN) stimulation on above responses or not and to analysis transmitter mechanism.</p><p><b>METHODS</b>Experiments were performed on white male rabbits anesthetised with chloralose and urethan. Experimental data were collected by means of stimulating Hb and DPN, recording arterial blood pressure and ectal electrocardiogram (EECG) and microinjecting transmitter blocker.</p><p><b>RESULTS</b>Prominent pressor and ischemic EECG segment changes were elicited by Hb stimulation (P < 0.01) Microinjecting kynurenic acid into ipsilateral DMH had partial blocking effect on pressor and ischemic EECG-ST segment changes induced by Hb stimulation (P < 0.01). Stimulation of bilateral DPN prominently inhibited the pressor and ischemic EECG-ST changes induced by Hb stimulation (P < 0.01, P < 0.05). Microinjecting naloxone into ipsilateral DMH decreased the inhibitory effect of DPN stimulation on above cardiovascular responses elicited by Hb stimulation (P < 0.01, P < 0.05).</p><p><b>CONCLUSION</b>Glutamic acid receptor in DMH is involved in pressor and ischemic changes induced by Hb stimulation. DMH and opiate peptide receptors in DMH are involved in the inhibitory effect of DPN stimulation on pressor and ischemic changes induced by Hb stimulation.</p>